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Monoclonal Antibodies Specific for Members of the Genus Endotrypanum

Identifieur interne : 00BD18 ( Main/Exploration ); précédent : 00BD17; suivant : 00BD19

Monoclonal Antibodies Specific for Members of the Genus Endotrypanum

Auteurs : Angela Hampshire C. S. Lopes [États-Unis, Brésil] ; Diane Mcmahon-Pratt [États-Unis]

Source :

The Journal of Protozoology [ 0022-3921 ] ; 1989-07.

RBID : ISTEX:47A811E796AD3C712E6A3FC336EFEBEC85C59D93

English descriptors

KwdEn :
- Antibody, Antigenic, Assay, Choloepus, Didactylus, Endotrypanum, Endotrypanum schaudinni, Endotrypanum species, Endotrypanurn, Endotrypanurn rnonterogeii, Endotrypanurn schaudinni, Endotrypanurn species, Endotrypanurn strain, Endotrypanurn strains, Fusion cxxx, Genus, Immunofluorescence, Immunoglobulin, Indirect radioimmune binding assay, Leishmania, Membrane preparations, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Negative control, Other strains, Other trypanosomatids, Parasite, Positive control, Radioimmune, Radioimmune assay, Sandfly, Schaudinni, Sloth, Trypanosoma, Trypanosomatidae, Trypanosomatidae family, Trypanosomatids, Western blot analysis.
Teeft :
- Antibody, Antigenic, Assay, Choloepus, Didactylus, Endotrypanum, Endotrypanum schaudinni, Endotrypanum species, Endotrypanurn, Endotrypanurn rnonterogeii, Endotrypanurn schaudinni, Endotrypanurn species, Endotrypanurn strain, Endotrypanurn strains, Fusion cxxx, Genus, Immunofluorescence, Immunoglobulin, Indirect radioimmune binding assay, Leishmania, Membrane preparations, Monoclonal, Monoclonal antibodies, Monoclonal antibody, Negative control, Other strains, Other trypanosomatids, Parasite, Positive control, Radioimmune, Radioimmune assay, Sandfly, Schaudinni, Sloth, Trypanosoma, Trypanosomatidae, Trypanosomatidae family, Trypanosomatids, Western blot analysis.

Abstract

Twenty‐six monoclonal antibodies were produced against membrane‐enriched preparations of Endotrypanum schaudinni or Endotrypanum sp. promastigotes. Fifteen of these monoclonal antibodies (E1‐E15) reacted only with the standard strain of E. schaudinni, M6159. Monoclonal antibodies E16‐E26 were considered Endotrypanum specific; no cross reactivity was detected with any other genus of the family Trypanosomatidae (Leishmania, Trypanosoma, Leptomonas. Herpetomonas or Crithidia) by dot‐blot radioimmune assay. By indirect immunofluorescence assay, the antigens recognized by Endotrypanum specific monoclonal antibodies appear to be associated with the surface of the parasite. Based on Western blot analysis, 4 antigenic molecules ranging in molecular weight from 24 kD to 160 kD were identified by monoclonal antibodies specific for the strain of E. schaudinni, M6159. Monoclonal antibodies specific for the genus Endotrypanum identified an antigen of molecular weight 48 kD as well as a diffuse component migrating with an apparent molecular weight of 64–200 kD.

Url:

https://api.istex.fr/document/47A811E796AD3C712E6A3FC336EFEBEC85C59D93/fulltext/pdf

DOI: 10.1111/j.1550-7408.1989.tb05526.x

Affiliations:

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Le document en format XML

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<term>Didactylus</term>
<term>Endotrypanum</term>
<term>Endotrypanum schaudinni</term>
<term>Endotrypanum species</term>
<term>Endotrypanurn</term>
<term>Endotrypanurn rnonterogeii</term>
<term>Endotrypanurn schaudinni</term>
<term>Endotrypanurn species</term>
<term>Endotrypanurn strain</term>
<term>Endotrypanurn strains</term>
<term>Fusion cxxx</term>
<term>Genus</term>
<term>Immunofluorescence</term>
<term>Immunoglobulin</term>
<term>Indirect radioimmune binding assay</term>
<term>Leishmania</term>
<term>Membrane preparations</term>
<term>Monoclonal</term>
<term>Monoclonal antibodies</term>
<term>Monoclonal antibody</term>
<term>Negative control</term>
<term>Other strains</term>
<term>Other trypanosomatids</term>
<term>Parasite</term>
<term>Positive control</term>
<term>Radioimmune</term>
<term>Radioimmune assay</term>
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<term>Sloth</term>
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<term>Endotrypanurn schaudinni</term>
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<term>Endotrypanurn strain</term>
<term>Endotrypanurn strains</term>
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<term>Immunofluorescence</term>
<term>Immunoglobulin</term>
<term>Indirect radioimmune binding assay</term>
<term>Leishmania</term>
<term>Membrane preparations</term>
<term>Monoclonal</term>
<term>Monoclonal antibodies</term>
<term>Monoclonal antibody</term>
<term>Negative control</term>
<term>Other strains</term>
<term>Other trypanosomatids</term>
<term>Parasite</term>
<term>Positive control</term>
<term>Radioimmune</term>
<term>Radioimmune assay</term>
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<term>Schaudinni</term>
<term>Sloth</term>
<term>Trypanosoma</term>
<term>Trypanosomatidae</term>
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<front><div type="abstract" xml:lang="en">Twenty‐six monoclonal antibodies were produced against membrane‐enriched preparations of Endotrypanum schaudinni or Endotrypanum sp. promastigotes. Fifteen of these monoclonal antibodies (E1‐E15) reacted only with the standard strain of E. schaudinni, M6159. Monoclonal antibodies E16‐E26 were considered Endotrypanum specific; no cross reactivity was detected with any other genus of the family Trypanosomatidae (Leishmania, Trypanosoma, Leptomonas. Herpetomonas or Crithidia) by dot‐blot radioimmune assay. By indirect immunofluorescence assay, the antigens recognized by Endotrypanum specific monoclonal antibodies appear to be associated with the surface of the parasite. Based on Western blot analysis, 4 antigenic molecules ranging in molecular weight from 24 kD to 160 kD were identified by monoclonal antibodies specific for the strain of E. schaudinni, M6159. Monoclonal antibodies specific for the genus Endotrypanum identified an antigen of molecular weight 48 kD as well as a diffuse component migrating with an apparent molecular weight of 64–200 kD.</div>
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Monoclonal Antibodies Specific for Members of the Genus Endotrypanum

Monoclonal Antibodies Specific for Members of the Genus Endotrypanum

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri